Formula is a special dietary food that simulates breast milk specifically for infants. Vitamins and other nutrients are added to baby formula to provide the essential nutrition for infants who are not breastfed or partially breastfed. Breast milk is the optimal source of nutrition for babies, as it contains a unique composition of nutrients that support growth, development, and immunity. Baby formula ingredients should be carefully chosen and regulated to match the nutritional composition and quality of breast milk as closely as possible.
How to determine the vitamins added into baby formula
In fact, for all baby formulas, different countries have different regulations to manage them. Mostly, these formulas need specific approval before entering into the market. The content of vitamins and other nutrients added shall also be well calculated and tested before selling. Currently, the determination of these added nutrients is based on AOAC, or ISO standards.
For examples,
- AOAC 960.46 describes microbiological test method for B12, folic acid, niacin, pentothenic acid & riboflavin.
- ISO 20634:2015 Infant formula and adult nutritionals Determination of vitamin B12 by reversed phase high performance liquid chromatography (RP-HPLC)
Alternately, there are also commercial test kits developed based on these standard methods for users to easily determine the target analytes.
Ringbio Enzymatic Assay Kits for vitamin determination.
Now there are 5 commercial kits available at Ringbio for the quick determination of vitamins, which include,
| Product Code | Product | Validation |
| R6001 | Biotin, vitamin B7 Rapid Test Kit | ILVO |
| R6002 | Folic acid, vitamin B9 Rapid Test Kit | ILVO |
| R6003 | Vitamin B12 Rapid Test Kit | ILVO |
| R6004 | L-carnitine Enzymatic Assay Kit | Internally |
| R6005 | Choline Enzymatic Assay Kit | Internally |
These test kits are widely in commercial baby formula manufacturers and have been validated internally and externally.
Besides these commercial kits, Ringbio has some important enzymes that are used in the determination of these vitamins, which can also be provided upon request. These enzymes are readily available and can be perfect replacement for other brands.
| Product Code | Product | Validation |
| ZB101 | Choline oxidase, recombinant, 1mg, lyophilized format * replace for Sigma C4405, C5896 |
Internally |
| ZB102 | Carnitine acetyltransferase, recombinant, 1mg, lyophilized format * replace for Roche/Sigma 10103241001 and Sigma C4899 |
Internally |
If you are interested, please contact us for details.
