African Swine Fever Virus antigen ASFV Ag Rapid Test Kit is used to detect ASF antigen in swine serum, plasma, and whole blood.
Basic information
African swine fever (ASF) is a severe viral disease affecting domestic and wild pigs. More recently (since 2007) the disease has been reported in multiple countries across Africa, Asia, and Europe, in both domestic and wild pigs. In 2018 and 2019, ASFV is spreading quickly to Asia, Southeast Asia. In countries like China, Vietnam, etc now ASFV is becoming a big threat to local animal farming.
This ASFV antigen rapid test kit is based on lateral flow immunoassay to detect antigen of ASFV in swine serum/plasma/tissue extract, etc. which only takes 10-15min to get a visible result. No special instrument required and no extra cost added, which is quite suitable for lab tests and field tests.
The antibodies used in this kit is a paired monoclonal antibody against ASFV p72, P30 and P54 protein. Sensitivity is also enhanced by mixed other ASFV specific antibodies.
Note: this kit does not contain any contagious materials, which is not hazardous to human or animal.
| Product name | ASFV Ag Rapid Test Kit |
|---|---|
| Product code | C30021 |
| Principle | Sandwich antibody immunoassay |
| Species | Swine, porcine |
| Specificy | >95% |
| Sensitivity | >90% compared with Real-time qPCR when testing the infected dead pigs. |
| Detection limit | 106 - 107 TCID50/ml, Ct value of qPCR is approx. 25 according to validation by EU Lab |
| Shelf-life | 12 months |
| Storage | 2- 30 oC or 2-8 oC |
* The detection limit of this kit was tested by the ASF Reference Laboratory of Canada
Key facts of the African Swine Fever Virus antigen ASFV Ag Rapid Test Kit
- Ready to use kits for farmers and the general lab staff
- No special instrument required
- Suitable for field test
- Result in 10-15min
- Detection limit: this kit detects ASF Ag to CT value of 30 or less according to comparison research done by our customers.
African Swine Fever Virus antigen ASFV Ag Rapid Test Kit Components
| Item # | Item | Qty |
|---|---|---|
| 1 | ASFV Ag Rapid Test Card | 50 pcs |
| 2 | Sample buffer, 5ml per bottle | 3 bottles |
| 3 | Plastic pipettes, 0.5ml | 50 pcs |
| 4 | Kit instruction, | 1 set. |
Video on how to operate the ASFV Ag Rapid Test Kit
Certificates
- This kit has been submitted for registration in the China Ministry of Agriculture and Rural Affairs
Citations
- Jarynowski, A., Płatek, D., Krzowski, Ł., Gerylovich, A., & Belik, V. (2019). African Swine Fever-potential biological warfare threat (No. 1904). EasyChair.
- Cavalera, Simone, et al. "Development of molecular and antigenic-based rapid tests for the identification of African swine fever virus in different tissues." Talanta 258 (2023): 124443.
- HA, Angi, and R. D. Tulle. "Seroprevalence Rate and Factors Causing African swine fever Outbreak in Kupang City and Regency of East Nusa Tenggara Province, Indonesia." (2023).
- Cavalera, Simone, et al. "Development of molecular and antigenic-based rapid tests for the identification of African swine fever virus in different tissues." Talanta 258 (2023): 124443.
