This Nucleic acid extraction kit is suitable for extracting nucleic acid (DNA/RNA) from whole blood, serum, plasma, liquid milk, body fluid, culture medium, tissue homogenate, mouth and nose, feces, environment, etc. The resulting DNA/RNA can be used directly in nucleic acid detection applications.
Basic information of the Nucleic acid extraction kit
The nucleic acid extraction kit is based on silica gel column purification technology, which eliminates the need for toxic phenol chloroform extraction or time-consuming alcohol precipitation. The silica membrane centrifugal adsorption column was used to specifically bind DNA/RNA, and impurities were removed by washing solution, and high-purity DNA/RNA was obtained by eluting in the eluent. The silica gel adsorption column specifically adsorbs DNA/RNA to minimize the removal of proteins and other impurities.
Key facts of the Nucleic acid extraction kit
- No toxic organic solvent required.
- High recovery of nucleic acid.
- Extraction in 30min.
Technical Specifications of the Nucleic acid extraction kit
| Product Code | TQ007 |
|---|---|
| Package Size | 50T, 200T |
| Principle | Silica gel column purification technology |
| Sample Application |
Serum, plasma, whole blood, liquid milk, body fluids, culture fluids, tissue homogenates, mouth and nose, feces, environmental and other swab samples. |
| Kit Component |
|
| Shelf-life | 12 months |
| Storage | 2-30°C |
| Delivery | Room temperature |
Sample preparation:
- Serum sample: centrifuge > 500 μL of whole blood for 30 s and take 200 μL of supernatant to be tested.
- Plasma sample: centrifuge > 500 μLEDTA anticoagulant for 30 s and take 200 μL of supernatant to be tested.
- Whole blood sample: mix well to be tested.
- Tissue sample: take 0.05-1g tissue sample, add 5-10 times normal saline and grind to homogenate, take 200μL of supernatant for testing.
- Swab sample: use a cotton swab to infiltrate in normal saline, then flip and apply at the sampling site, after completing the collection, break the swab head and put it into a centrifuge tube containing 1mL of normal saline and vortex for 30s, take 200μL of mixing solution to be tested.
- Note: If the sample mixture has obvious particles, centrifuge at 12000r/min for 30~60s to remove impurities before proceeding to the next step.
