Antibiotic residue is used to describe the harmful substances that remain in milk and other animal products after the treatment of animals with antibiotics. These substances can cause health problems for humans and animals, such as allergic reactions, antibiotic resistance, and environmental pollution. Some of the common antibiotics that are found in milk are β-lactam, tetracycline, fluoroquinolone, sulfonamide, and aminoglycosides, etc.
The current Accuplus MAT5 test kit is a microbial inhibition test kit for broad-spectrum antibiotic residue testing in milk. It is based on the inhibition of bacteria growth by antibiotic residues in milk to detect if the residual level of these chemicals is below the safe level (MRL).
Key facts of the Accuplus MAT5 Test Kit
- Ready to use kits for lab staff.
- No organic solvent required.
- Result visible in 2.5hours.
- Broad spectrum detection for more than 34 kinds of antibiotics.
Detection Limit of the Accuplus MAT5 Test Kit
The kit is developed to meet EU MRLs, and this detection limit may change, please refer to the kit insert.
Kit Components
- Precoated microtiter plate, 5 plates, in total 480 reactions
- Plate cover film, 5 pcs
- Color card, 1 set
- Kit instruction manual
Materials required but not provided.
- Micropipette, 20-200ul
Sample preparation
- Milk samples are tested directly.
Demonstration Video
Brief test procedure
Please add 200ul milk into the microwell, and then incubate for 2.5 hours at 64degree celsius. Positive and negative control shall also be operated at the same time to ensure test accuracy.
Reference
- Suhren, G., & Heeschen, W. (1996). Detection of inhibitors in milk by microbial tests. A review. Die Nahrung, 40(1), 1–7. https://doi.org/10.1002/food.19960400102
- BAM Chapter 20A: Inhibitory Substances in Milk, https://www.fda.gov/food/laboratory-methods-food/bam-chapter-20a-inhibi…
